Chromatographic separation of 14 different flavonoids by TLC
Summary
Type: TLC Application #: 400680
Detection: UV 254 + 366 nm
or spray with 1% methanolic solution of ethanolamine diphenylborate, then with 5% ethanolic solution of polyethylene glycol, finally view under UV at 360 nm (for spray reagents see ch Conditions: Eluents :
1) acetic acid / methanol / water (3:3:4, v/v)
2) 0.1 M ammonia in 55% methanol
3) hexane / ethyl acetate / acetic acid (72:27:1, v/v)
See also : D. Heimler, V. Boddi, J. Chromatogr. 4 Authors: Heimler D Source: J Chromatogr 366 (1986) 407